When colon cancer cell lines HCT116wt and HCT116neg were treated with PRIMA-1Met, The phosphorylation of downstream molecules of MEK such as ERK1/2 and RSK2 was dramatically attenuated with PRIMA-1Met concentration more than 25 uM (Figure 3C, 3D). This evidence concerns the gene MAP2K7 and malignant colon neoplasm.