After the transfection of BX357664, cells were stimulated with recombinant human transforming growth factor-beta 1 (Rh TGF-β1) to upregulate the protein level of TGF-β1 to verify if tumor behavior could be rescued by restoring TGF-β1 expression in the presence of BX357664 overexpression. The gene discussed is TGFB1; the disease is neoplasm.