2013). In our study, DMSO treatment induced gluconeogenesis in a time‐dependent manner, as indicated by increased PEPCK and G6Pase expression. Carbohydrate metabolism differs in hepatoma cells compared to primary hepatocytes as the former maintain energy production through anaerobic glycolysis (Hugo‐Wissemann et al. 1991). Here, PCK2 is linked to hepatocellular carcinoma.