CD34 and acute myeloid leukemia: To determine the potential effects of miR-99a upregulation on cellular function of LSCs, primary CD34+ cells were isolated from two AML patients with a low expression level of miR-99a (AML2 and AML6, as shown in Figure 1C) then transduced with lentivirus carrying hsa-miR-99a-5p (miR-99a) or a scrambled sequence (Ctrl), tagged with enhanced green fluorescent protein (eGFP) (Figure S2A), followed by the colony forming cell (CFC) assay.