Retrospective analysis comparing responses to early infection (3 WPI) by non-vaccinates versus culture-negative vaccinates (n = 14) or culture-positive vaccinates (n = 5) revealed differential IFN-γ/TNF-α responses by Tcm cells to ESAT-6:CFP10 among the groups (Figure 10A, P < 0.05). This evidence concerns the gene IFNG and infection.