TNF and infection: Importantly, both IFN-γ/TNF-α/IL-2 and IFN-γ/TNF-α responses to infection at 3 WPI (data not shown) and 8 WPI (Figure S7 in Supplementary Material) exceeded (P < 0.05) respective responses to vaccination upon recall stimulation of long-term cultures with Ag85A/TB10.4.