To do this, total PBMCs, pDC-depleted PBMCs, and pDCs isolated from four individual donors were independently treated with serially diluted GS-9620 for 36 h, and then cell-free culture supernatants were transferred to isolated autologous CD4+ T cells 24 h prior to infection with HIV-1VSV-G-LUC. The gene discussed is CD4; the disease is infection.