CD4 and HIV infectious disease: Likewise, the TLR7 agonists imiquimod and gardiquimod also showed no significant direct antiviral activity in autologous CD4+ T cells (EC50s = 9.4 ± 4.5 μM and 5.1 ± 0.4 μM, respectively; CC50s = 9.6 ± 1.7 μM and 7.0 ± 1.0 μM, respectively) but strongly suppressed HIV infection in isolated CD4+ T cells upon the addition of conditioned cell-free PBMC supernatants treated with imiquimod and gardiquimod (EC50s = 100 ± 30 nM and 40 ± 35 nM, respectively) (Fig. 2).