We observed that upon withdrawal of temsirolimus treatment, there was a resumption of tumor growth in the PDX model (Fig. 4e) with consequent increase in proliferative activity (Fig. 4d), decrease in apoptosis (Fig. 4d), and re-activation of mTOR pathway signaling (Fig. 4b and Additional file 1: Figure S4B). The gene discussed is MTOR; the disease is neoplasm.