Presence of high evening postprandial insulin response, and HOMA-IR score in our study during acute one-day exposure to 60% carbohydrate diet in metabolically healthy, glucose tolerant individuals raises the question of whether this effect may become clinically significant with extended exposure to this diet in view of the health risks of chronic hyperglycemia and hyperinsulinemia of Type 2 diabetes [2–6]. This evidence concerns the gene INS and type 2 diabetes mellitus.