Three in vivo and in vitro models were used in this study, a well-characterized murine model of experimental allergic conjunctivitis (EAC) induced by SRW pollen in BALB/c, TLR4 deficient (Tlr4-d) and MyD88 knockout (MyD88−/−) mice, a topical ocular surface challenge murine model, and a culture model of primary human corneal epithelial cells (HCECs) exposed to an aqueous extract of defatted SRW pollen (SRWe). Here, MYD88 is linked to atopic conjunctivitis.