Our findings also provide physiological and molecular evidence that DMY improves insulin resistance in HFD-fed rats by (i) stimulating GLUT1 translocation from the cytosol to the membrane through the activation of AMPK signaling to promote glucose uptake, and (ii) regulating the expression of G6Pase and PEPCK via the IRS/PI3K/Akt pathways to decrease glucose production. Here, IARS1 is linked to Insulin resistance.