In this study, we have found that JB downregulated the mRNA expression of glucose transporter genes (Glut1, Glut3 and Glut4) and glycolysis-related kinase genes (Hk2 and Ldha), increased ROS level and decreased the potential of mitochondrial membrane, and subsequently induced tumor cells apoptosis in B16F10 cells. This evidence concerns the gene SLC2A3 and neoplasm.