As shown in Figure 3A and 3C, we found that the mRNA expressions of S100A4 is significantly and positively correlated with Nanog in both sphere cells and head and neck cancers, consistent with our prior finding that demonstrates the co-expression of S100A4 and Nanog in HNSCC patients [26]. The gene discussed is S100A4; the disease is head and neck squamous cell carcinoma.