To determine the role of c-Myc in the chemosensitivity of 5-FU in colon cancer cells in hypoxia, HCT116 and SW480 cells were transfected with empty control vector or c-Myc expression plasmid (Figure 4B), treated with 5-FU, and then cultured under normoxic or hypoxic conditions (2, 8 and 24 hours). This evidence concerns the gene MYC and malignant colon neoplasm.