In order to determine the dynamic expression changes of c-Myc as a function of time in hypoxia, colon cancer cells (HCT116 and SW480) were cultured in normoxic (21% O2) or hypoxic (1% O2) conditions for 0.5, 1, 2, 4, 8, 18 and 24 hours. The gene discussed is MYC; the disease is malignant colon neoplasm.