To further investigate the potential role of Twist1 in radioresistance of NPC, we engineeringly generated Twist1 stably expressing CNE2 cell line (CNE2-T) and Twist1 knockdown CNE2-R cell line (CNE2-R-shTwist1) (Supplementary Figure S1, B-C). Here, TWIST1 is linked to nasopharyngeal carcinoma.