Using small interfering RNAs (siRNAs) and gene overexpression, we next investigated the effects of modulating GLI1, GLI2, and GLI3 on the expression of the known Hedgehog target gene, PTCH1, and KIT. Due to GIST-T1 cells' resistance to electroporation, we focused on the GIST882 cell line. The gene discussed is PTCH1; the disease is gastrointestinal stromal tumor.