Similarly, the NID2 promoter is also methylated in the ESCC cell lines (KYSE30, KYSE150, and SLMT-1S1), while the NID2 promoter in NE3 and NE083 (immortalized esophageal cell lines) remain unmethylated (Supplementary Figure S3). Here, NID2 is linked to esophageal squamous cell carcinoma.