BRAF and hematologic disorder: While in literature data concerning the sensitivity of the QT-PCR and the dd-PCR in HCL are few, analytic sensitivity tests have been performed at the start of the study, diluting a DNA from a HCL patient with 5% of B-RAF mutation in a pool of 5 wild-type DNAs (extracted from patients without hematological diseases during orthopedic surgery) in a half-logarithmic scale, from 1 × 10 to 1 × 10-4 dilution.