To determine MYH6 association with HLHS, a case-control analysis for rare, damaging MYH6 variants was performed using a cohort of 190 unrelated HLHS subjects, all of whom had undergone either WES or WGS, with the Thousand Genomes Phase1v3 database serving as a control cohort (See supplemental methods, next-generation sequencing). Here, MYH6 is linked to hypoplastic left heart syndrome.