To evaluate the effects of PA-MSHA on EGFR signaling in pancreatic cancer cells, we examined the expression of key downstream proteins in the EGFR pathway (t-EGFR, t-AKT, t-ERK, p-EGFR, p-AKT, and p-ERK) by western blotting. Here, AKT1 is linked to familial pancreatic carcinoma.