TP53 and Zika virus infectious disease: To validate this prediction, we performed P53 immunodetection in human NSCi90 hNPCs, under control conditions or after infection with the French Polynesian variant of ZIKV, which has been associated with microcephaly.42, 43 Increased nuclear accumulation of P53 is known to correlate with P53 activation.44, 45 ZIKV infection significantly enhanced the number of cells with P53-positive nuclei (Figures 2a and b), as well as the average nuclear P53 intensity (Figure 2b).