However, we showed that in TRPML1-knockdown mammalian cells or mucolipidosis IV human fibroblasts, the inhibited mTORC1 signaling was only partially reversed by leucine or overexpression of constitutively active Rag GTPase, suggesting that there is a difference in the mechanisms of regulation of mTOR by Ca2+/CaM between mammalian and fly cells. Here, MCOLN1 is linked to mucolipidosis type IV.