2.5 × 105 EO771 tumor cells were injected orthotopically into the 4th fat pads (murine mammary glands) of wild type (WT) and caspase-1 knockout (KO) female mice (all on the C57BL/6 background) at about eight weeks old (Fig. 1), then tumor growth was measured once every two days. The gene discussed is CASP1; the disease is neoplasm.