In the present study, we have performed a comparative transcriptome analysis and quantitative PCR (qPCR) for expression profiling of the whole reticulon family in cultured human hepatoma and mouse fibroblast cell lines and primary mouse neurons, and show that NOGO-B/RTN4B is the main RTN4 isoform expressed in hepatoma and fibroblast cells and Nogo-A/Rtn4A in primary neurons. The gene discussed is RTN4; the disease is hepatocellular carcinoma.