In agreement with previous reports [22, 24], we found that first signs of amyloidosis and gliosis were detectable in the lower cortex and the corpus callosum of 2.5-month-old 5xFAD mice, although area fractions of GFAP staining and Iba-1 staining did not differ significantly from those of age-matched controls. The gene discussed is AIF1; the disease is amyloidosis.