EGFR and colorectal carcinoma: The calculated overall RAS mutation prevalence in this study was consistent with findings from sequenced CRC tumours in the 2012 TCGA database (49 %) and from a recent study of the reproducibility of RAS testing among pathology centres in the Netherlands (47.6 %), but was slightly lower than in a recently published pooled analysis of clinical trials of anti-EGFR therapy in patients with mCRC, which showed an overall RAS mutation prevalence of 55.9 % (95 % CI: 53.9–57.9 %) [23–25].