The WST-1 assay in combination with the CompuSyn algorithm and western blot analyses were utilized to compute and validate the combination indices (CI) of combined-treatment effect of Enzalutamide (MDV3100, AR antagonist), Dovitinib (TKI-258, pan RTK inhibitor), Buparlisib (BKM120, pan PI3K inhibitor), Everolimus (RAD001, mTOR inhibitor) and chemotherapy drug Cabazitaxel in different PCa cell lines. This evidence concerns the gene MTOR and posterior cortical atrophy.