The WST-1 assay in combination with the CompuSyn algorithm and western blot analyses were utilized to compute and validate the combination indices (CI) of combined-treatment effect of Enzalutamide (MDV3100, AR antagonist), Dovitinib (TKI-258, pan RTK inhibitor), Buparlisib (BKM120, pan PI3K inhibitor), Everolimus (RAD001, mTOR inhibitor) and chemotherapy drug Cabazitaxel in different PCa cell lines. The gene discussed is AR; the disease is posterior cortical atrophy.