Furthermore, in vitro culture of miR-155 KO CD4+ T cells with IL-4 stimulation resulted in enhanced Th2 differentiation, accompanied by elevated levels of Th2 cytokines (such as IL-4, IL-5, and IL-10), indicating that miR-155 may negatively regulate Th2 immune responses and inhibit the occurrence of inflammation in allergic diseases [21]. This evidence concerns the gene IL10 and allergic disease.