Next, to further clarify the role of IFN-γ and NK cells in MSCs-Sirt1-induced immunopotentiation to inhibit breast tumor growth in vivo, anti-asialoGM1 antiserum was utilized to inhibit the production of NK cells, and anti-IFN-γ antibody was also applied to realize IFN-γ depletion in tumor-bearing mice. Here, SIRT1 is linked to breast neoplasm.