As an example, an elevated methylation level was detected in 75% of the tumor samples and 0% of the normal lung samples for the HOXB3/HOXB4 MS-HRM assay, as shown in Fig. 1c, and in 87.9% of the tumor samples and only 3.2% of the normal lung samples for the OSR1 MS-HRM assay shown in Fig. 1i. The gene discussed is HOXB4; the disease is neoplasm.