To address the issue whether the MALAT1 combined with HULC influences on liver cancer stem cells malignant proliferation, we established the stable liver cancer stem lines transfected with pCMV6-A-GFP, pCMV6-A-GFP-HULC, pCMV6-A-GFP-MALAT1, pCMV6-A-GFP-HULC plus pCMV6-A-GFP-MALAT1. The gene discussed is MALAT1; the disease is liver cancer.