To test whether SRC‐3‐mediated ESCC pathogenesis was through IGF/AKT signaling pathway, mRNA and protein levels of numerous well‐known genes of IGF/AKT signaling pathway were compared between control and SRC‐3 knockdown cells by qPCR and Western blot analysis. This evidence concerns the gene AKT1 and esophageal squamous cell carcinoma.