Further Cox multivariate regression model demonstrated that CPA4 level (HR = 1.780; 95% CI: 1.090–2.908; P = 0.021), Grade (HR = 2.267; 95% CI: 1.369–3.725; P = 0.001), Distant metastasis (HR = 3.207; 95% CI: 1.121–9.179; P = 0.030),and lymph node metastasis (HR = 3.428; 95% CI: 1.565–7.509; P = 0.002) were statistically independent predictive factors of poorer prognosis for CRC cancer (Table 2). The gene discussed is CPA4; the disease is metastatic malignant neoplasm in the lymph nodes.