Therefore, to address whether low VB12 and VB9, confirmed by elevated homocysteine content, correlates with the expression of p53 in megaloblastic anemia, we have measured the levels of vitamin-B9, B12 and homocysteine in the serum of non-MBA control subjects and MBA patients, and quantified the p53 expression using immunohistochemistry of bone marrow biopsies embedded in paraffin blocks. Here, TP53 is linked to megaloblastic anemia.