GPR133-KD no. 1 GBM cells had reduced proliferation measured by Ki-67 immunostaining under both normoxia (GBML20: 0.67±0.02 of control) and hypoxia (GBML20: 0.56±0.10 of control) (Figures 4e and f). The gene discussed is ADGRD1; the disease is glioblastoma.