AHCY and hepatocellular carcinoma: Its increased expression has been found in hepatocellular carcinoma.24 ARK2 cells were again incubated with metformin (or control) in the presence or absence of the pharmacological SAHH inhibitor DEA.8 QMSP analysis was performed 48 h later using primers that specifically amplify a single differentially methylated region (DMR) of the genes chosen for further study.