The mRNA levels of M2 markers (Arg-1, Ym-1) increased at 24 h, peaked at 3 d after stroke and then began to decrease; while the mRNA levels of M1 markers (CD16, CD86) gradually increased over time from 3 d and remained elevated for at least 14 days after tMCAO28. The gene discussed is ARG1; the disease is stroke disorder.