EHEC infection also caused an increase of ERK1/2 phosphorylation in only 20% at 0.5 h of infection and co-stimulation with EGF after EHEC infection only increased 50–55% at 0.5, 1, and 2 h of infection and 30% at 4 h in comparison with EGF alone (100%; Figure 5B). This evidence concerns the gene EGF and infection.