Under dot blot conditions, which negate alterations in the electrophoretic mobility of tau between samples, due to different degrees of protein phosphorylation [17] and allows for quantification of total tau species phosphorylated at each investigated epitope, AT8 immunoreactivity was strongly enhanced in AD diagnosed cases, reporting a ~14-fold increase compared to non-AD samples (Fig. 2a i + ii, p < 0.001). Here, MAPT is linked to Alzheimer disease.