Mechanism of AGE-RAGE signaling in altering steroidogenesis in PCOS has been studied by Diamanti-Kandarakis et al. [84] in human ovarian granulosa cells (KGN) in which they cultured KGN granulosa cell line with LH alone and LH along with human glycated albumin (HGA, representative of AGEs) for 0–2 h. This evidence concerns the gene AGER and polycystic ovary syndrome.