In the present study, ENO1 rs2274971A > G, PFKM rs11168417C > T, PFKP rs1132173C > T, and PDK2 rs3785921G > A polymorphisms in glycolytic pathway were associated with survival outcomes after surgery, suggesting that the SNPs in glycolytic pathway may be prognostic markers in early stage NSCLC. This evidence concerns the gene ENO1 and non-small cell lung carcinoma.