Deficiencies at the network level have also been observed in other neurodevelopmental disorders, including in Tuberous Sclerosis Complex (TSC), which is caused by loss-of-function mutations in the mTOR negative regulators TSC1 or TSC2. In Tsc1 KO neuronal cultures, network hyperexcitability was attributed to a primary imbalance in excitation and inhibition due to reduced inhibition onto pyramidal neurons, but not to alterations in homeostatic excitatory synaptic plasticity33. This evidence concerns the gene TSC2 and tuberous sclerosis.