Additionally, TUNEL assays were carried out and the results showed that a greater number of tumor cells underwent apoptosis in mice treating with VNP-pN-FADD and VNP-pN-N-FADD compared with mice receiving VNP or VNP-pN (Fig. 6B), suggesting that FADD or N-FADD overexpression in tumor tissues could induce more melanoma cells to undergo apoptosis and VNP-pN-N-FADD induced the most number of tumor cells to undergo apoptosis among all of the recombinant VNP strains. This evidence concerns the gene FADD and melanoma.