Although antitumor proteins expressed and delivered by attenuated S. typhimurium without any secretion peptides or CPPs may influence the efficiency release of the proteins into tumor cells more or less, our results showed that N-FADD delivered by VNP under the control of NirB promoter (VNP-pN-N-FADD) was successfully expressed in tumor sites and significantly suppressed the growth of tumor and prolonged survive time of tumor-bearing mice compared with other engineered strains (P < 0.05) (Fig. 5). Here, FADD is linked to neoplasm.