In this study, we demonstrated that nirB promoter-driven expression of FADD (VNP-pN-FADD) or its variant N-FADD (VNP-pN-N-FADD) by VNP could markedly suppressed melanoma growth, even more, VNP-pN-N-FADD with a more potent anti-tumor efficacy and markedly extended the survival of mice bearing melanoma than VNP-pN-FADD (Fig. 5). This evidence concerns the gene FADD and melanoma.