It was found that the re-inoculated tumours in mice after their first tumours were ablated by PLGA-ICG-R837-based PTT plus CTLA4 blockade therapy showed inhibited growth (Fig. 5b), and anti-CTLA4 treatment in the first round (after PTT ablation of first tumours) appeared to be not necessary to induce the immune-memory effect. This evidence concerns the gene CTLA4 and neoplasm.