It was discovered that M2 macrophage was remarkably able to promote proliferation (Figure 3B–3D), invasion (Figure 3E–3F) and migration (Figure 3G–3H) of glioma cells U271 and U87 in comparison with co-culture with THP-1 treated with macrophage colony stimulating factor (M-CSF), indicating that M2 macrophages induced from monocytes were capable of promoting proliferation, invasion and migration of glioma cells in vitro. This evidence concerns the gene CSF1 and glioma.