It was discovered that M2 macrophage was remarkably able to promote proliferation (Figure 3B–3D), invasion (Figure 3E–3F) and migration (Figure 3G–3H) of glioma cells U271 and U87 in comparison with co-culture with THP-1 treated with macrophage colony stimulating factor (M-CSF), indicating that M2 macrophages induced from monocytes were capable of promoting proliferation, invasion and migration of glioma cells in vitro. This evidence concerns the gene CSF1 and central nervous system cancer.