MCF7 tumours isolated from the preventative group were too small to allow ex-vivo analysis, therefore the anti-tumour effects of anakinra were assessed in isolated MDA-MB-231-IV tumours: Tumour cell apoptosis, assessed following immunohistochemistry for active Caspase 3, was not altered following daily injection with anakinra (Figure 5c and 5g), however necrotic cell death was significantly increased (P < 0.01 for treatment and P<0.001 for preventative compared with placebo) (Figure 5d). The gene discussed is CASP3; the disease is neoplasm.