Since the MEK/ERK cascade is aberrantly activated in RMS and its inhibition has been shown to affect tumour growth and to rescue skeletal muscle differentiation in ERMS cells [38], as occurred in si-DNMT3B transfected cells, we studied if DNMT3B expression could be linked to the MEK/ERK signalling pathway. This evidence concerns the gene DNMT3B and embryonal rhabdomyosarcoma.