Four human PCa cell lines (LNCaP, LNCaP C4-2B [C4-2B], PC-3, PC-3M) previously reported to have progressively increasing metastatic capacity [25–28] were characterized for epithelial (E-cadherin/EpCAM/CK) and mesenchymal (N-cadherin/vimentin) markers using qRT-PCR, immunoblotting (Supplementary Figure S1A, 1B), and flow cytometry (FCM) (Figure 1A). Here, VIM is linked to posterior cortical atrophy.