Overall, the results show that MYCN mainly functions as a repressor of the expression of differentiation-inducing miRNAs in neuroblastoma cells by negatively regulating the expression of six differentiation-inducing miRNAs (miR-449b-5p, miR-137, miR-124-3p, miR-34a-5p, miR-449a and miR-506-3p) in at least one of the three cell lines. Here, MYCN is linked to neuroblastoma.