Flow cytometry analysis showed that JQ1 alone did not have an effect, that vincristine alone slightly increased the proportion of neuroblastoma cells positively stained by the apoptosis marker Annexin-V, and that JQ1 and vincristine synergistically and significantly increased the proportion of BE(2)-C and Kelly neuroblastoma, but not WI38 embryonic fibroblast (<10%), cells positively stained by Annexin-V (Figure 5C and Supplementary Figure S7). This evidence concerns the gene ANXA5 and neuroblastoma.