ESR1 and breast neoplasm: Similarly, enrichment of ERα and H3K27Ac mark was also found around the HYAL3 gene (HYAL3 is encoded by the bottom DNA strand), but this region does not seem to confer estrogen activation of HYAL3 since no significant regulation in expression was observed in treated MCF-7 cells (Figure 2B and Supplementary Figure S3) and ESR1 and HYAL3 gene expression did not correlate in breast tumors (Table 1).